Density | 1.020g/mLat 20°C(lit.) |
Flash Point | 40°C |
Appearance | White or white-like powder or bead powder |
Storage Condition | 2-8°C |
Hazard Symbols | Xi - Irritant |
Risk Codes | R10 - Flammable R36/37/38 - Irritating to eyes, respiratory system and skin. |
Safety Description | S24/25 - Avoid contact with skin and eyes. S22 - Do not breathe dust. S36/37 - Wear suitable protective clothing and gloves. S26 - In case of contact with eyes, rinse immediately with plenty of water and seek medical advice. S16 - Keep away from sources of ignition. |
UN IDs | UN 1170 3/PG 3 |
WGK Germany | 3 |
FLUKA BRAND F CODES | 10-21 |
Use | agarose protein A is A gel, mainly used in scientific research. |
preparation | in the experiment, the AFB1 Immunoaffinity column was successfully prepared by using agarose protein A gel as the ligand to couple the monoclonal antibody. The coupling rate of MAB was 98.1%. 48 μl of purified antibody was used for coupling, and the average relative column capacity was 105ng/0.125mL gel. The column blank test showed that the agarose protein A blank gel had no non-specific adsorption to afb1. The addition and recovery experiments of different samples with different mass concentrations show that the smaller column volume can meet the purification of AFB1 in the sample. The purity of the monoclonal antibody is not required for coupling. From the results of electrophoresis of the reaction solution after coupling, it can be seen that the band is A impurity protein, and all the effective monoclonal antibodies are coupled to protein A, which reveals that the method of coupling monoclonal antibodies does not require high purity, because of the specific binding of agarose protein A to IgG1, the octanoic acid-saturated ammonium sulfate preliminary purified antibody can meet the coupling requirements. |